SCANNING ELECTRON MICROSCOPY TO CONFIRM THE EFFICACY OF MEASUREMENT OF HAIR THICKNESS BY CONVENTIONAL METHODS
- Abstract number
- 783
- Event
- European Microscopy Congress 2020
- DOI
- 10.22443/rms.emc2020.783
- Corresponding Email
- [email protected]
- Session
- LSA.12 - Application of EM in health industry
- Authors
- dr. Koray Erdogan (1), PROF. DR ALP BAYRAMOGLU (3), DR. ELIF NEDRET KESKINOZ (3), ASSIST.PROF. DR. MERVE ELMAS (4), PROF. DR. SERAP ARBAK (4), prof. dr. mutlu HAYRAN (2)
- Affiliations
-
1. ASMED Hair Transplant Clinic
2. Department of Preventive Oncology, Hacettepe University Hospital.
3. School of Medicine, Department of Anatomy, Acibadem Mehmet Ali Aydinlar University
4. School of Medicine, Department of Histology and embryology , Acibadem Mehmet Ali Aydinlar University
- Keywords
Hair transplantation, FUE, SEM, caliber device
- Abstract text
Objective: The extraction/excision follicular unit (FUE) is known to be an easy procedure to perform and it is a sensitive process that is vulnerable to several forms of injury. The success of FUE procedures is affected by various factors. Hair thickness, density and average hair number per follicle, are the important factors for the success of FUE. Knowing these data is extremely important for the acceptable coverage of the bald area. It is insufficient and various information about the hair thickness in the literature. Currently, hair diameter is mesured with a caliber device that compresses the hair follicle and measures its cross-sectional reagion. In this study, we aim to evaluate hair diameter in men with scanning electron microscopy (SEM). For this aim, hair follicles from each donor area (occipital, temporal, parietal) were collected. Hair follicle diameter and thickness from each region were measured. Each result taken from SEM was compared with the results of caliber device.
Methods: Hair samples were taken from occipital, temporal and parietal region. For each regional examination, 90 samples were analyzed. Totally 270 specimens were used for the analysis of hair follicle measurements. Samples were mounted on aluminum pin stubs with double-sided adhesive carbon disks prior to analysis under the SEM. Samples were observed under a scanning electron microscope (Thermo Scientific- Quattro SEM) using GSED dedector. The short and long transverse axes of each hair shaft were examined and then photographed at 2,000 magnification. Micrographs were overlaid with measurement data and scale bars. Data was analyzed statistically.
Results: A correlation has been prominently determined between the measurements of hair follicle thickness of each donor area with SEM and caliber device in each. The measurement of short axes taken from caliber device has been correlated with SEM measurements. Hair thickness from occipital reagion was gradually decreased from temporal to occipital reagion in all donor area.
Conclusion: This study revealed that the caliber device used for the measurements showed reliable results as a diagnostic instrument to measure the thickness of hair. Compared with the SEM measurement results, the device itself showed a high degree of precision and accuracy for the measurements.
- References
1. Azar, R.P., et al., Alterations in Hair Follicle Morphology and Hair Shaft Production After Follicular Unit Transplantation. Am J Dermatopathol, 2016. 38(10): p. 732-8.
2. Hirano-Ali, S.A., et al., Scanning Electron Microscopic Hair Shaft Analysis in Ectodermal Dysplasia Syndromes. Pediatr Dermatol, 2015. 32(6): p. 836-44.
3. Mohmand, M.H. and M. Ahmad, Transection rate at different areas of scalp during follicular unit extraction/excision (FUE). J Cosmet Dermatol, 2019.